Custom Oligonucleotide Synthesis
Research and Development of ASOs, siRNA, and PMOs
OLIGO Services
Custom Oligo Synthesis Services
Sinopep's oligonucleotide CRDMO platform, situated in Hangzhou Biopharma Town, which boasts a highly qualified research team, with more than half of its members holding master's degrees or higher. The platform has achieved significant milestones in oligonucleotide development, including the successful synthesis of various types of oligos such as ASO, SiRNA, and PMO. Additionally, the platform has collaborated with international companies to produce the first generic drug of oligonucleotides, marking a groundbreaking achievement.
We offer research-grade and clinical-grade oligonucleotide manufacturing services, supporting clients from early discovery through clinical development. Utilizing solid- and liquid-phase approaches, we provide custom oligo contract development and manufacturing, including scale-up production and comprehensive analytical testing.
Oligonucleotide Types
Full-spectrum custom synthesis capabilities supporting diverse therapeutic modalities, delivering high-purity ASOs, modified siRNAs, and conjugated architectures optimized for therapeutic development.
| Oligo Type | Supported Variants | Mechanism & Significance |
|---|---|---|
| Antisense (ASO) | Gapmers, Mixmers | RNase H-mediated mRNA cleavage & pre-mRNA splicing modulation. |
| siRNA | Duplex siRNA, asymmetric siRNA | RISC-mediated catalytic mRNA degradation for post-transcriptional silencing. |
| miRNA | Agomirs, Antagomirs | Endogenous microRNA regulation via direct mimicry or steric inhibition. |
| Morpholinos (PMO) | PMO, PPMO (Peptide-conjugated) | Uncharged backbone preventing enzymatic cleavage for exon skipping. |
| Aptamers | DNA Aptamers, RNA Aptamers | High-affinity molecular targeting against specific proteins and cell receptors. |
| CpG Oligos | Class A, Class B, Class C CpG ODNs | TLR9 receptor activation to stimulate innate and adaptive immune responses. |
| Decoy Oligos | Double-stranded DNA decoys | Competitive binding to transcription factors to inhibit pathogenic gene expression. |
| Peptide-Oligo Conjugates | POCs, Cell-Penetrating Peptides | Enhanced biological membrane transit and targeted tissue internalization. |
| tRNA & gRNA | sgRNA, crRNA, tracrRNA | CRISPR-Cas genome editing complexes and translational Machinery support. |
| Circular & Branched Oligos | circRNA mimics, Dendromers | Exonuclease degradation resistance and high-density functional group display. |
Oligonucleotide Modificatons
Precision chemical modification platforms engineered to optimize metabolic stability, enhance cellular targeting, and maximize therapeutic efficacy across custom oligonucleotide sequences.
| Modification Category | Available Options | Key Technical Benefit |
|---|---|---|
| Base & Sugar Modifications | 2′-OMe, 2′-MOE, 2′-F, 2′-LNA, cEt, 5mC, dU, dI, Pseudouridine (Ψ), N1-methyl-Ψ | Duplex stability, nuclease resistance, cross-linking, immune evasion |
| Backbone Linkages | Phosphorothioate (PS) bonds, Phosphorodithioate (PS2), Mesyl phosphoramidate | Exonuclease & endonuclease protection |
| Phosphorylation & Cap Modifications | 5′ Phosphorylation, 3′ Phosphorylation, 5′-Vinylphosphonate (5′-VP), Inverted dT (3′-3′) | Ligase substrate (5′), extension blocking (3′), RNAi activity, terminal cap degradation resistance |
| Targeting & Lipid Conjugates | GalNAc (tri-antennary), Cholesterol, Fatty Acids (C16, C18, C22), Tocopherol, Folate | Hepatic tissue delivery, cellular uptake, endosomal escape, extended circulation |
| Linkers | Amino modifiers (C3, C6, C12), Biotin, Biotin TEG, Thiol groups (S-S), Carboxyl, Maleimide | Covalent attachment & conjugation |
| Spacers | C3, Spacer 6, Spacer 9, Spacer 12, Spacer 18, dSpacer (Abasic), PC Spacer | Spatial distance control, abasic site mimicking, photocleavable release |
| Click Chemistry Handles | Alkyne, Azide (N3), DBCO, DBCO-PEG4, TCO, Tetrazine (TZ) | Selective, aqueous-phase labeling without side reactions |
| Fluorophores | FAM, HEX, TET, Cy3, Cy3.5, Cy5, Cy5.5, Cy7, TAMRA, ROX, Texas Red, ATTO dyes | qPCR, FISH, smFRET, & sequencing detection assays |
| Quenchers & Non-Fluorescent Labels | BHQ-1, BHQ-2, BHQ-3, DABCYL, Eclipse, MGB (Minor Groove Binder), DNP | Fluorescence quenching, duplex stabilization (MGB), hybridization specificity |
| Polymer Conjugation | PEGylation (PEG3, PEG4, PEG6, PEG12, PEG24, linear/branched PEG) | In vivo stability, solubility, reduced renal clearance |
Years Experience
Employees
Oligo Capacity (MT/yr)
GMP Faclities
Oligo Quality Tests
We provide all in-house release testing for typical GMP- and research-grade product specifications.
| Category | Method | Instrument | Test Description & Purpose |
|---|---|---|---|
| Appearance | Visual Appearance | N/A | Evaluates color, clarity, and physical state of the solution or lyophilized powder. |
| General Properties | pH (1% w/v solution) | Mettler Toledo S470-K/S400-K | Measures acidity/alkalinity to confirm formulation stability and compatibility. |
| Osmolality | YASN Osmo210 | Determines solute concentration to ensure isotonicity for biological applications. | |
| Water Determination | Mettler Toledo C30 | Karl Fischer titration to quantify residual moisture content in solid samples. | |
| Identity | Single-strand molecular mass by IPRP-MS | Waters Acquity Premier / Xevo G3 QTof | Accurate mass verification of full-length single-stranded oligonucleotides. |
| Tm by UV spectroscopy | Thermo E201 | Determines melting temperature to assess duplex thermal stability and hybridization. | |
| Sequencing by MS/MS | Waters Acquity Premier / Xevo G3 QTof | Tandem mass spectrometry fragmentation to confirm primary nucleotide sequence. | |
| Duplex retention time | Agilent 1260/1290, Waters Acquity Premier | Chromatographic profiling to verify duplex formation and hybridization integrity. | |
| Counter Ions | Sodium content by IC | Thermo ICS 6000 | Ion chromatography quantification of sodium salt form counter-ions. |
| Assay | UV absorption (anhydrous) | Thermo E201, NanoDrop | Spectrophotometric quantification of active oligonucleotide content at 260 nm. |
| Purity | Non-denaturing IPRP-HPLC-UV | Waters Acquity Premier, QTof G3 | Assesses intact secondary structure purity and native double-strand percentage. |
| Denaturing AEX-HPLC UV | Waters Acquity Premier, QTof G3 | Anion-exchange separation resolving full-length product from short-mer failure sequences. | |
| Denaturing IPRP-HPLC UV | Waters Acquity Premier, QTof G3 | Ion-pair reversed-phase resolution of lipophilic impurities and modified variants. | |
| Process Impurities | Residual solvents (GC-FID) | Agilent 8890B GC | Gas chromatography profiling to ensure organic solvent levels meet ICH limits. |
| Elemental impurities (ICP-MS) | Thermo iCAP RQ | Trace heavy metal analysis (e.g., Pd, As, Cd, Pb) via plasma mass spectrometry. | |
| Safety | Bacterial endotoxins | Microplate Reader | LAL assay detection to confirm pyrogen-free status for biological safety. |
| Safety | Bioburden | Microplate Reader | Quantitative microbial enumeration test ensuring sterility standards. |
